Meiji Techno TC5000 Series Instrukcja Obsługi Pobierz pdf (Strona 25)

3.3 Incident Light Operation: Epi-Fluorescence

For microscopes with fluorescence option installed only.

For viewing of transparent objects using incident-light fluorescence, we recommend to

first make your initial adjustments using brightfield with transmitted light.

1. First, switch on the power supply to the Nobska lamphouse and allow the lamp to

achieve operating temperature. (Operating temperature is achieved when the power

supply settles on the proper operating voltage. See your Nobska Operations Manual for

more details.)

2. Open the light stop leading to the

lamphouse.

3. Move filter block into the light

beam path.

4. Remove the objective currently in

the light path.

5. Place a sheet of white paper on the

specimen stage.

6. Observe if the arc image is clearly

projected and centered in the light spot as shown in Figure X. (If not, proceed to the

section in the Nobska Lamphouse Manual describing Mercury Lamp arc position

adjustment.)

7. Remove the paper and re-install the objective.

8. Position the specimen on the stage.

9. Using a low power objective, check to see if the image is illuminated evenly. If not,

adjust the settings on the Nobska Lamphouse if needed.

Be careful not to project the arc reflection onto the arc electrodes. This will cause an

over-heating condition followed by an explosion of the mercury lamp.

The two electrodes of the arc can be seen in the extension of the plane of the discharge

arc. Refer to the Nobska Lamphouse Manual for further information and adjustment

instructions.

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Meiji Techno TC5000 Series Instrukcja Obsługi Strona 25